Definitions

1. Primers were derived from a region of the small RNA segment of Lassa virus coding for the glycoprotein (36). (Web site)
2. Primers (and the master mix) are added - the PCR protocol has to be started with an extended time at 95^^C. RACE-PCR - Rapid amplification of cDNA ends.
3. Primers are short, artificial DNA strands--not more than fifty nucleotides Since DNA is usually double-stranded, its length is measured in base pairs. (Web site)
4. Primers were selected in conserved gene regions for the amplification of a fragment comprising about two thirds of the rnpB gene in streptococci. (Web site)
5. Primers were designed to amplify the region between the last 100 nucleotides of cinA and the first 100 nucleotides of cinQ, spanning the intragenic space.

Carinii Strains

1. X13687), enables the design of primers and probes which can differentiate between P. carinii strains. (Web site)

Unextended Primers

1. Accordingly, a number of different techniques can be used to facilitate the removal of unextended primers.

Caga Gene

1. The cagA gene was analyzed using the primers D008 and R008 [ 19]. The cagE gene was analyzed using the primers described by Fallone et al.

Universal Primers

1. Extracted DNA from gingival margin plaque was subjected to PCR targeting the 16S rRNA gene using universal primers. (Web site)

Fluorescently

1. Visualization can either be through the use of fluorescently tagged dyes in the primers or by silver staining the gel prior to scanning. (Web site)

Pgmy Primers

1. Enhanced detection and typing of human papillomavirus (HPV) DNA in anogenital samples with PGMY primers and the linear array HPV genotyping test. (Web site)
2. Use of PGMY primers in L1 consensus PCR improves detection of human papillomavirus DNA in genital samples. (Web site)

Oxide Primers

1. Products include stains, varnishes, enamels, oxide primers, and UV curable coatings. (Web site)

Probes Specific

1. Primers and probes specific for human P. carinii 18S ribosomal RNA gene sequences are provided. (Web site)

Novel Primers

1. We have deduced novel primers for general amplification of the s and m regions from sequences obtained from European H. pylori strains. (Web site)
2. Therefore, it is assumed that these novel primers and probes will bind with high likelihood to unknown virus isolates. (Web site)

Conserved Region

1. The primers are based on 30 to 90 sequences of a conserved region in each virus. (Web site)

Standard Pcr

1. Because the rDNA sequence information used to develop the primers and probes was based upon that used for standard PCR for An. (Web site)

Msg Primers

1. Using PCR analysis with MSG primers, they also detected P. carinii DNA in blood samples from their immunosuppressed rats.

Species Specific Primers

1. In the latter case, this may be achieved by using genus specific primers or species specific primers derived from the ITS region of Serratia species. (Web site)

Rapid Amplification

1. For 3′ rapid amplification of cDNA ends (3′ RACE), primer UAP (3′ RACE kit), and the sam1 gene-specific primers were used.

Gel

1. The primers and primer dimers migrate out of the gel and do not interfere with final staining analyses. (Web site)

Oligonucleotides

1. DNA fragments were ligated to oligonucleotides that served as sites for PCR primers.

Specific Pcr Primers

1. Specific PCR primers and probes can be designed based on the variable regions of this molecule to detect certain species or groups of bacteria.

Putative Sequence Specific Pcr Primers

1. Haugland R A, Heckman J L. Identification of putative sequence specific PCR primers for the detection of the toxigenic fungal species Stachybotrys chartarum.

Oligonucleotide Primers Ptp1

1. PCR was done using oligonucleotide primers PTp1 and PTp2 which amplify a 191-base pair DNA fragment of pertussis toxin operon. (Web site)

Rcc Primers

1. This amplification yielded 360-bp products that included the region to be amplified by the Rcc primers. (Web site)
2. Rcc primers were also used to amplify DNA template with an excess of P. carinii f. (Web site)

Dna Target

1. For example, unextended primers can be removed from SBE reactions that are run with small amounts of DNA target.

Horizontal Arrows

1. The horizontal arrows indicate the locations of the primers used in PCR, which are described in Table 2. (Web site)
2. The horizontal arrows represent PCR primers used for controlling the genetic structure of the recombinant strains. (Web site)

Nested Primers

1. No. 4,683,195, which describes the use of nested primers for increasing PCR sensitivity in the amplification of single copy genes. (Web site)
2. PCR analysis consisted of two rounds of PCR using nested primers. (Web site)

Gene-Specific Primers

1. When mice were infected with isolate BC7, a PCR with gene-specific primers for cblA (48) was used to confirm the identity of the recovered bacteria.

Type-Specific Primers

1. Typing hepatitis C virus by polymerase chain reaction with type-specific primers: application to clinical surveys and tracing infectious sources. (Web site)

Flanking Regions

1. The PCR primers were designed to target the flanking regions of each deletion so that the amplified region spanned the missing locus.

Pcr Primers Specific

1. Furthermore, a set of PCR primers specific for B. edulis was designed for establishing a convenient and accurate method of identifying this edible mushroom. (Web site)
2. PCR primers specific for the genus Tuber reveal the presence of several truffle species in a truffle-ground.

Random Primers

1. Poly (A+) RNA was isolated from activated THP-1 cells, and cDNA synthesis was initiatedusing oligo(dT) and random primers. (Web site)

Primers Specific

1. A 324-bp product indicative of the cag PAI was obtained with primers specific for the cagA gene from each of the 31 strains. (Web site)
2. For each isolate, four different targets were amplified by PCR, using primers specific for Echinococcus DNA (see procedures in Haag et al. (Web site)
3. Using arbitrarily primed RT-PCR (4, 9, 10) and RT-PCR with primers specific for the coronavirus family (7, 8), cDNA fragments were generated and sequenced. (Web site)

Degenerate Primers

1. The other use for degenerate primers is when primer design is based on protein sequence. (Web site)

Specific Primers

1. Expression of each subunit in mouse tissue was verified by RT-PCR using specific primers and the AccessQuick™ RT-PCR System.
2. Mix cDNA with qPCR reagents, then aliquot a standard volume of this "master mix" into each reaction well containing a standard volume of specific primers. (Web site)
3. Specific primers amplify conserved region in H. pylori genome.

Oligonucleotide Primers

1. Using RT-PCR and oligonucleotide primers for pituitary GH cDNA, GH mRNA was shown to be present in the testes and vas deferens of adult cockerels. (Web site)
2. In the presence of an excess of dNTPs (the 'building blocks' of new DNA material), oligonucleotide primers are added. (Web site)
3. The oligonucleotide primers may be provided suspended in an aqueous solution or as a freeze-dried or lyophilized powder, for instance. (Web site)

Study

1. Primers used for amplifying and sequence the Delphinidae mtDNA in this study.
2. Table 1. PCR primers used in this study.

Combination

1. Similarly, the VANS1 primer has been used in combination with other primers to amplify rDNA from vesicular-arbuscular endomycorrhizal fungi (27). (Web site)
2. A combination of two random primers (OPD18 and OPAA17) used in separate reaction tubes identified 23 of the 25 dermatophyte species or subspecies examined. (Web site)

Sense

1. Sequencing was accomplished with both sense and antisense nested PCR primers; only the sense direction is shown.

Outer

1. The complete cocktail of primers for each outer and inner primer pair were then used together for each of the four amplifications targeting the four viruses. (Web site)
2. The first- and second-round PCR were performed using outer and inner adapter primers along with the sam1 gene-specific primers.

Choice

1. PCR reaction, the choice of oligonucleotide primers is critical.

Interest

1. We sequenced the areas around the microdeletion and the stop codons by PCR using primers that flanked these areas of interest.
2. Variable and constant domains can be cloned, e.g., using the polymerase chain reaction and primers which are selected to amplify the domain of interest. (Web site)

Portion

1. The primers and probes used to amplify and detect the portion of the HFE gene around codon 282.

Developed

1. Primers and fluorescence resonance energy transfer probes were developed that target the cdc2 gene of P. jiroveci. (Web site)
2. Primers and probes of species present indoors will be developed for rapid QPCR diagnosis.

Clinical Specimens

1. Clinical specimens were screened for influenza A virus by multiplex real-time PCR and then subtyped as H1 or H3 with specific primers. (Web site)

Variety

1. Includes stains, primers, and intermediate and top coats in variety of formulations. (Web site)

Internal

1. Potential bias of fungal 18S rDNA and internal transcribed spacer polymerase chain reaction primers for estimating fungal biodiversity in soil.

Procedure

1. The procedure involves the use of two oligonucleotide primers which flank the DNA fragment to be amplified. (Web site)

Required

1. If required to improve specificity, two different pairs of primers may be used.
2. Another common use for degenerate primers is required when primer design can only be performed on protein sequence.

Mutation

1. Here, one of the two primers is common, and would anneal a short distance away from the mutation, while the other anneals right on the variation. (Web site)
2. The sequence is modified by substituting (or mismatching) a base near the mutation of interest in one of the two PCR primers. (Web site)

Categories

1. Pcr
2. Amplification
3. Probes
4. Polymerase Chain
5. Science > Biology > Genetics > Dna

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