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Ethidium Bromide       Article     History   Tree Map
  Encyclopedia of Keywords > Chemistry > Biochemistry > Molecular Biology > Agarose Gel Electrophoresis > Ethidium Bromide   Michael Charnine

Keywords and Sections
ETHIDIUM
PCR AMPLICONS
PRODUCTS
ADDITION
PRESENCE
ALTERNATIVES
PROCESS
GLOVES
VISUALIZATION
BUFFER
CARCINOGENIC
DOXORUBICIN
RNA
AGAROSE
ULTRAVIOLET
ULTRAVIOLET LIGHT
GELS
RESEARCH LABORATORIES
ACRIDINE
DISPOSAL
INTERCALATION
NUCLEIC
SYBR GREEN
MUTAGENIC
GEL
UV ILLUMINATION
MUTAGEN
DNA FRAGMENTS
ETBR
CARCINOGEN
DYE
ELECTROPHORESIS
DNA SAMPLES
SOLUTION
SOAKING
GEL ELECTROPHORESIS
NUCLEIC ACIDS
DNA
AGAROSE GELS
ETHIDIUM BROMIDE STAINING
STAIN
STAINING
UV LIGHT
STAINED
PCR PRODUCTS
AGAROSE GEL ELECTROPHORESIS
Review of Short Phrases and Links

    This Review contains major "Ethidium Bromide"- related terms, short phrases and links grouped together in the form of Encyclopedia article. Please click on Move Up to move good phrases up.

Definitions Submit/More Info Add a definition

  1. Ethidium Bromide (EB) is the most commonly used DNA intercalator, both in gel and capillary electrophoresis.
  2. Ethidium bromide (EB) is a mutagen and toxin that is widely used in the laboratory for visualization of nucleic acids. Move Up
  3. Ethidium Bromide is a fluorescent dye that is commonly added to agarose gels. Move Up
  4. Ethidium bromide is a potential carcinogen: wear gloves and handle with care. Move Up
  5. Ethidium bromide is a potent mutagen and should be handled with care. Move Up

Ethidium Submit/More Info Add phrase and link

  1. I always loaded ethidium into gel, about 0.4 microliters of Ethidium Bromide per 25 ml of gel.

Pcr Amplicons Move Up Add phrase and link

  1. The PCR amplicons were subjected to 2% agarose gel electrophoresis, followed by staining with ethidium bromide.

Products Move Up Add phrase and link

  1. The amplified products were separated on a 1.5% agarose gel and visualized by staining with ethidium bromide and were subjected to DNA sequencing.

Addition Move Up Add phrase and link

  1. The DNA is visualised in the gel by addition of ethidium bromide.

Presence Move Up Add phrase and link

  1. Agarose gel electrophoresis of linear genomic DNA in the presence of ethidium bromide: band shifting and implications for forensic identity testing.

Alternatives Move Up Add phrase and link

  1. Alternatives to ethidium bromide are available.

Process Move Up Add phrase and link

  1. Ethidium bromide, silver, or coomassie blue dye may be used for this process.

Gloves Move Up Add phrase and link

  1. Remember to wear gloves when handling any solution containing ethidium bromide, which is a potent mutagen and potential carcinogen.

Visualization Move Up Add phrase and link

  1. Visualization of larger amounts of DNA requires less sensitivity and is often possible using stains such as ethidium bromide.
  2. Visualization of Band Migration When the gel is prepared, it is usually mixed with a DNA dye known as ethidium bromide. Move Up

Buffer Move Up Add phrase and link

  1. The fragments are fluorescently labeled using ethidium bromide in the running buffer and detected with a laser-excited, confocal-fluorescence scanner.

Carcinogenic Move Up Add phrase and link

  1. Ethidium bromide is very carcinogenic.

Doxorubicin Move Up Add phrase and link

  1. Most intercalators are aromatic and planar molecules; examples include ethidium bromide, daunomycin, and doxorubicin.

Rna Move Up Add phrase and link

  1. Note: This solution contains ethidium bromide that can interfere with transfer of RNA to nylon or nitrocellulose membranes.

Agarose Move Up Add phrase and link

  1. Following size fractionation on an approximate gel matrix, for example, agarose or acrylamide, the gel is soaked in a dilute solution of ethidium bromide.

Ultraviolet Move Up Add phrase and link

  1. Alleles were separated on 3-6% MetaPhor agarose gels, stained with ethidium bromide, and visualized by ultraviolet (UV) illumination.

Ultraviolet Light Move Up Add phrase and link

  1. The DNA is stained with ethidium bromide, and is then visible under ultraviolet light.
  2. It is now possible to visualize the DNA (stained with ethidium bromide) with ultraviolet light. Move Up

Gels Move Up Add phrase and link

  1. Gels were stained with ethidium bromide and destained with water before photography.
  2. Methods include radiolabeling (exposure of gels to x-ray film) and various stains (ethidium bromide, silver stains etc.). Move Up
  3. Unbound ethidium bromide was removed by washing the gels in deionized water for 60 min. Move Up

Research Laboratories Move Up Add phrase and link

  1. Research laboratories use ethidium bromide, an ultraviolet (UV) fluorescent stain, because it shows very small amounts of DNA and is faster to use.

Acridine Move Up Add phrase and link

  1. Examples include ethidium bromide and acridine.
  2. Monomers can also be intercalating agents such as acridine, thiazole organge, or ethidium bromide. Move Up

Disposal Move Up Add phrase and link

  1. These loading dyes reduce exposure and many of the problems associated with ethidium bromide use and disposal.
  2. The disposal of laboratory Ethidium bromide remains a controversial subject [ 16]. Move Up

Intercalation Move Up Add phrase and link

  1. Ethidium bromide adheres to the DNA by intercalation between the base pairs of the double helix structure of the DNA.

Nucleic Move Up Add phrase and link

  1. Such effects may also be enhanced by addition of intercalators which intercalate into the double helix of the nucleic acid, such as ethidium bromide.
  2. The agarose solution is mixed with ethidium bromide, which will bind to the nucleic acid during the electrophoresis procedure. Move Up

Sybr Green Move Up Add phrase and link

  1. In addition, PicoGreen, like ethidium bromide, is an intercalating dye and its safety will need to be compared to that of SYBR Green I.

Mutagenic Move Up Add phrase and link

  1. Ethidium bromide has been shown to be mutagenic to bacteria via the ames test (with liver homogenate) [ 9].

Gel Move Up Add phrase and link

  1. The pattern of fragments is visualized and photographed under ultraviolet illumination by staining the gel with ethidium bromide.
  2. Put the gel, together with the rack, into a tank with 0.5x TBE. Ethidium bromide at the same concentration can be added to the buffer. Move Up
  3. In research laboratories, ethidium bromide and similar fluorescent compounds such as Acridine Orange are normally used to visualise DNA on a gel. Move Up

Uv Illumination Move Up Add phrase and link

  1. The gels are then examined under UV illumination after staining with ethidium bromide.
  2. Only the marker dyes can be seen The gel with UV illumination, the ethidium bromide stained DNA glows orange Digital photo of the gel. Move Up

Mutagen Move Up Add phrase and link

  1. NOTE: Ethidium bromide is a known mutagen and should be handled as a hazardous chemical - wear gloves while handling.

Dna Fragments Move Up Add phrase and link

  1. The DNA fragments of different lengths are visualized using a fluorescent dye specific for DNA, such as ethidium bromide.
  2. When adequate migration has occured, DNA fragments are visualized by staining with ethidium bromide. Move Up

Etbr Move Up Add phrase and link

  1. Typically, ethidium bromide (EtBr) has been used as the staining agent.

Carcinogen Move Up Add phrase and link

  1. Ethidium bromide may be a mutagen, carcinogen or teratogen although this depends on the organism and conditions employed.

Dye Move Up Add phrase and link

  1. An agarose gel containing separated DNA fragments stained with a dye (ethidium bromide) and visualized under ultraviolet light.

Electrophoresis Move Up Add phrase and link

  1. After electrophoresis of total RNA samples in the presence of ethidium bromide, the 28S and 18S human rRNA should be clearly visible under UV illumination.

Dna Samples Move Up Add phrase and link

  1. DNA samples are stained during electrophoresis by ethidium bromide in the running buffer.

Solution Move Up Add phrase and link

  1. Gels were stained in ethidium bromide solution for 10 minutes, destained in water for 5 minutes, and visualized.
  2. Stir the solution to disperse the ethidium bromide, then pour it into the gel rack. Move Up
  3. The presence of ethidium bromide in the solution allows for RNA visualization without additional staining of denaturing agarose gels. Move Up

Soaking Move Up Add phrase and link

  1. The banded DNA is then detected by soaking the gel briefly in a solution containing a dye called ethidium bromide (EtBr).
  2. Some researchers prefer not to add ethidium bromide to the gel itself, instead soaking the gel in an ethidium bromide solution after running. Move Up
  3. Microwave oven, casting tray, ethidium bromide (or soaking after electrophoresis), sample combs, cooling 5. Move Up

Gel Electrophoresis Move Up Add phrase and link

  1. The PCR amplicons were visualized by gel electrophoresis on a 1% agarose gel stained with ethidium bromide.
  2. The most convenient method to visualize DNA in gel electrophoresis is staining with the fluorescent dye ethidium bromide. Move Up

Nucleic Acids Move Up Add phrase and link

  1. Originally, SDE gels were stained with ethidium bromide (EtBr) given its widespread use as an intercalating fluorescent dye used to detect nucleic acids.
  2. Nucleic acids that are separated in a gel can be seen with ethidium bromide or other stains. Move Up
  3. Because ethidium bromide also binds with nucleic acids, caution is advised in its handling and disposal. Move Up

Dna Move Up Add phrase and link

  1. REPLAY ANIMATION Step 5: Staining the DNA bands - The DNA is visualized by binding to ethidium bromide (EtBr) as it migrates through the gel.
  2. These gels are visualized on a U.V. trans-illuminator by staining the DNA with a fluorescent dye (ethidium bromide). Move Up
  3. Consequently, ethidium bromide is often used as a marker for apoptosis in cells populations and to locate bands of DNA in gel electrophoresis. Move Up

Agarose Gels Move Up Add phrase and link

  1. Agarose gels with Ethidium Bromide must be disposed of as hazardous waste.
  2. The most convenient and commonly used method to visualize DNA in agarose gels is staining with the fluorescent dye ethidium bromide. Move Up

Ethidium Bromide Staining Move Up Add phrase and link

  1. DNA bands were visualized and photographed in a Fluor-S MultiImager (BioRad) after ethidium bromide staining.
  2. Reaction products were resolved in an agarose gel and subsequently visualized by ethidium bromide staining. Move Up
  3. Every animal that had received at least 10 4 P. carinii organisms produced a PCR product detectable by ethidium bromide staining (Table 3). Move Up

Stain Move Up Add phrase and link

  1. Five dyes offered superior sensitivity in the detection and quantification of DNA, over Ethidium Bromide, the most commonly used nucleic acid stain.
  2. Stain the gel with ethidium bromide. Move Up
  3. Thus, SYBR Green I stain is a weak mutagen and appears to be much less mutagenic than ethidium bromide. Move Up

Staining Move Up Add phrase and link

  1. Ethidium bromide staining is commonly used to visualize RNA or DNA in agarose gels placed on UV light boxes.
  2. PCR products were analyzed by electrophoresis in 1.2% agarose gels and visualized after ethidium bromide staining. Move Up
  3. The reaction products were analyzed by agarose gel electrophoresis followed by ethidium bromide staining. Move Up

Uv Light Move Up Add phrase and link

  1. After being stained with ethidium bromide, the bands in the gel were visualized and photographed under UV light.
  2. Ethidium Bromide: Intercalates within the structure of nucleic acids in such a way that they fluoresce under UV light. Move Up
  3. However, ethidium bromide is a powerful mutagen (and probably a carcinogen) and UV light can cause serious eye and skin burns. Move Up

Stained Move Up Add phrase and link

  1. After electrophoresis, the gels were stained with ethidium bromide and were photographed under UV light at 302 nm.
  2. After gel electrophoresis, the gels were stained with ethidium bromide and were photographed under UV light at 302 nm. Move Up
  3. DNA and RNA are generally stained with ethidium bromide (EtBr), an intercalating agent. Move Up

Pcr Products Move Up Add phrase and link

  1. PCR products were run on agarose gels and visualized with ethidium bromide to confirm the presence of a band of the expected size.
  2. PCR products were visualized under UV illumination after electrophoresis migration on a 1% agarose gel stained with ethidium bromide. Move Up
  3. PCR products were separated by electrophoresis in 1.2% agarose, and stained with ethidium bromide (19). Move Up

Agarose Gel Electrophoresis Move Up Add phrase and link

  1. Visualisation: EtBr & dyes The central dye in agarose gel electrophoresis is ethidium bromide, usually abbreviated as EtBr.
  2. PCR products were subjected to agarose gel electrophoresis and stained with ethidium bromide. Move Up
  3. If a conventional PCR is used, the PCR product is detected using agarose gel electrophoresis and ethidium bromide (or other nucleic acid staining). Move Up

Categories Submit/More Info

  1. Chemistry > Biochemistry > Molecular Biology > Agarose Gel Electrophoresis
  2. Stained Move Up
  3. Staining Move Up
  4. Pcr Products Move Up
  5. Uv Light Move Up
  6. Books about "Ethidium Bromide" in Amazon.com

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  Short phrases about "Ethidium Bromide"
  Originally created: August 01, 2010.
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